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KMID : 0895820120220020105
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Journal of Oriental Rehabilitation Medicine
2012 Volume.22 No. 2 p.105 ~ p.118
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Effects of Gamchobuja-tang on the Adjuvant-induced Arthritis in Rats
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Choi Jin-Seo
Jeong Su-Hyeon
Seo Il-Bok
Kim Soon-Joong
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Abstract
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Objectives :This study was performed to investigate the effects of Gamchobuja-tang(gancaofu□zˇl-tang) on the Complete Freund`s Adjuvant(CFA)-induced arthritis in rats.
Methods :Arthritis was induced by intradermal injection of CFA into the base of tail. Arthritic rats were divided into control(n=8) and Gamchobuja-tang(gancaofu□zˇl-tang) treated(n=8) group. Control group was taken distilled water for 15 days. Gamchobuja-tang(gancaofu□zˇl-tang) treated group was taken extracts of Gamchobuja-tang(gancaofu□zˇl-tang) by orally for same duration. Normal group(n=8) was injected with light mineral oil and was taken distilled water. Gross structure of hind paw were observed at every 5 days after treatment. Body weight, paw edema volume and ankle joint thickness were measured at 0, 5, 10, 15th day after treatment. Histopathological examination on the tarsal joint and distal tibia of the right hind paw were performed at the 15th day after treatment. White blood cell counts in blood were analysed at the 15th day after treatment. The content of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in paw exudate were analysed by enzyme-linked immunosorbent assay(ELISA) at the 15th day after treatment.
Results :1. Grossly, the severity of arthritis in the Gamchobuja-tang(gancaofu□zˇl-tang) treated group were alleviated compared with control group. 2. Paw edema volume(ml) of Gamchobuja-tang(gancaofu□zˇl-tang) treat group was significantly decreased compared with control group. 3. Ankle joint thickness(mm) of Gamchobuja-tang(gancaofu□zˇl-tang) treat group was significantly decreased compared with control group. 4. Histopathologically, destructive lession of synovial membrane, articular cartilage and bony tissue in Gamchobuja-tang(gancaofu□zˇl-tang) treated group were alleviated compared with control group. 5. Total WBC(103/μl) of Gamchobuja-tang(gancaofu□zˇl-tang) treated group was significantly decreased compared with control group. 6. TNF-α content(pg/ml) in paw exudation of Gamchobuja-tang(gancaofu□zˇl-tang) treat group was significantly decreased compared with control group.
Conclusions :On the basis of these results, Gamchobuja-tang(gancaofu□zˇl-tang) has anti-arthritic effects on the progression of CFA-induced arthritis in rat. And it`s effect is related with reduced secretion of TNF-α.
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KEYWORD
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Gamchobuja-tang(gancaofu□zˇl-tang), Adjuvant arthritis, WBC, TNF-α
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